11/24/2023 0 Comments 10x chromium droplet based![]() For isolated nuclei 25,000 reads each are recommended, For most applications, an average of 40,000 reads per cell should be sequenced (for cell types with complex transcriptomes). ![]() (The assay requires at least a 28 cycle forward read, a 10 bp dual index reads, and a 90 cycle reverse read). The 10X GEX Single-Cell libraries are most economically sequenced on the Illumina NovaSeq 6000 with paired-end reads.
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